......... Elektronová mikroskopie pro biology .........
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Proč elektrony?
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     elektronový
     mikroskop (TEM)
Příprava preparátů
     pro TEM chemickou
     cestou
Příprava preprátů
     pro TEM fyzikálními
     metodami
Další metody přípravy
     prepárátů pro TEM
Skanovací elektronový
     mikroskop (SEM)
Příprava preparátů
     pro SEM
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Proč elektrony?
Williams D.B., Carter C.B. 1996: Transmission Electron Microscopy, Plenum Press. New York
Dawes C.J. 1988: Introduction to Biological Electron Microscopy: Theory and Techniques
Ladd Research Industries, Inc. Publisher Burlington, Vermont
Kalina T., Pokorný V. 1981: Základy elektronové mikroskopie pro biology. Univerzita Karlova, Praha

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TEM
Williams D.B., Carter C.B. 1996: Transmission Electron Microscopy, Plenum Press. New York
Dawes C.J. 1988: Introduction to Biological Electron Microscopy: Theory and Techniques Ladd Research Industries, Inc. Publisher Burlington, Vermont
Kalina T., Pokorný V. 1981: Základy elektronové mikroskopie pro biology. Univerzita Karlova, Praha
Hulínský V., Jurek K. 1982: Zkoumání látek elektronovým paprskem. SNTL Praha
Ludvík J.: Elektronová mikroskopie. V: Prosser V. a kol. 1989: Experimentální metody biofyziky. Academia Praha
Carlemalm E., Colliex C., Kellenberger E. 1985: Contrast formation in electron microscopy of biological material. Adv. Imaging Electron Phys. 63: 269-334

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Příprava preparátů pro TEM chemickou cestou
Robards A.W., Wilson A.J. 1993: Procedures in Electron Microscopy, Willey and Sons,Ltd.
Mráz P., Polónyi J. 1988: Metody elektronovej mikroskopie živočíšnnych tkanív. Veda, Bratislava
Kalina T., Pokorný V. 1981: Základy elektronové mikroskopie pro biology. Univerzita Karlova, Praha
Dawes C.J. 1988: Introduction to Biological Electron Microscopy: Theory and Techniques Ladd Research Industries, Inc. Publisher Burlington, Vermont
Robinson D.G., Ehlers U., Herken R., Herrmann B., Mayer F., Schurmann F.-W. 1987: Methods of Preparation for Electron Microscopy. Spribger-Verlag, Berlin
Maunsbach A.B., Afzelius B.A. 1999: Biomedical Electron Microscopy. Academic Press
eid N., Beesley J.E. 1991: Sectioning and Cryosectioning for Electron Microscopy. Elsevier Amsterdam
Acetarin J. D., Carlemalm E., Kellenberger E. and Villiger W. 1987: Correlation of some mechanical properties of embedding resins with their behaviour in microtomy. J. Electron Microsc. Tech. 6: 63-79.
Afzelius B.A. 1992: Section Staining for Electron Microscopy Using Tannic Acid as a Mordant: A Simple Method for Visualization of Glycogen and Collagen. Microsc. Res. Tech. 21: 65-72.
Aimar P., Lossi L., Merighi A. 1997: Immunogold labelling for transmission electron microscopy: exploring new frontiers. Cell Vision 4: 394-407.
Baschong W., Prescianotto C. B., Wurtz M., Carlemalm E., Kellenberger C., Kellenberger E. 1984: Preservation of protein structures for electron microscopy by fixation with aldehydes and/or OsO4. Eur. J. Cell Biol. 35: 21-26.
Bendayan M., Nanci A., F. W. K. Kan 1987: Effect of Tissue Processing on Colloidal Gold Cytochemistry. J. Histochem. Cytochem. 35: 983-996.
Bijovsky A.T., Abrahamsohn P.A. 1991: Improved fixation for cytochemical demonstration of acid phosphatase in mouse decidual cells. J Electron Microsc. Tech. 17: 469-470.
Bullock G. R.1984: The current status of fixation for electron microscopy: a review. J. Microscopy 133: 1-15.
Coetzee J., van der Merwe C. F. 1987: Some characteristics of the buffer vehicle in glutaraldehyde - based fixatives. J. Microsc. 146: 143-155.
Disharoon D. R., Wickham M. G., Worthen D. M., Lofftus F. H. 1983: Vitreous carbon: A new material for making microtome knives. Stain Technol. 58: 143-151.
Emerman M., Behrman E. J. 1982: Cleavage and cross-linking of proteins with osmium (VII) reagents. J. Histochem. Cytochem. 30: 395-7.
Erickson P. A., Anderson D. H., Fisher S. K. 1987: Use of Uranyl Acetate En Bloc To Improve Tissue Preservation and Labeling for Post-Embedding Immunoelectron Microscopy. J. Electron Microsc. Tech. 5: 303-314.
Glauert A. M. and Hall C. 1991: Epoxy resins: an update on their selection and use. Eur. J. Microsc.13: 13-18.
Hobot J. A. and Newman G. R. 1991: Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy. Scanning Microsc. 5: S27-S41.
Jesior J.C. 1985: Direct Determination of Ultrathin Section Thickness Using Latex Particles. J. Electron Microsc. Tech. 2: 161-165.
Jésior J.C. 1986: How to Avoid Compression II. The Influence of Sectioning Conditions. J. Ultrastruct. Mol. Struct. Res. 95: 210-217.
Karnovsky M.J. 1965: A Formaldehyde-Glutaraldehyde Fixative of High Osmolality for Use in Electron Microscopy. Abstracts Fifth Meeting American Society for Cell Biology. J. Cell Biol. 27: 137A-138A.
Kellenberger E. 1998: Learning about truth and biases through experience: section surface corrugation, protein denaturation, and staining. Microsc. Res. Techn. 42: 33-42.
Kellenberger E., Villiger W., Carlemalm E. 1986: The Influence of the Surface Relief of Thin Sections of Embedded, Unstained Biological Material on Image Quality. Micron and Microsc. Acta 17: 331-348.
Lea P.J., Hollenberg M.J., Temkin R.J., Khan P.A.1992: Chemical Extraction of the Cytosol Using Osmium Tetroxide for High Resolution Scanning Electron Microscopy. Microsc. Res. Tech. 22: 185-193.
Luther P.K. 2001: Section Shrinkage: Impact for Routine and 3D Electron Microscopy. Microscopy and Analysis 70: 7-9.
Mollenhauer H. H. 1993: Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy. Microsc. Res. Tech. 26: 496-512.
Mollenhauer H. H. and Droleskey R.E. 1985: Some characteristic of epoxy embedding resins and how they affect contrast, cell organelle size, block shrinkage. J. Electron Microsc. Tech. 2: 557-62.
Mollenhauer H. H. and Bradfute O. E. 1987: Comparison of Surface Roughness of Sections Cut by Diamond, Sapphire, and Glass Knives. J. Electron Microsc. Tech. 6: 81-85.
Morgenstern E.1991: Aldehyde fixation causes membrane vesiculation during platelet exocytosis: A freeze-substitution study. Scanning Microsc., Supplement 5: S109-S115
Mori B., Tani G.: Soft dehydration in the embedding method for transmission electron microscopy. Caryologia 41: 181-189.
Murray A. B., Schulze H., Blauw E. 1991: In Situ Embedding of Cell Monolayers Cultured on Plastic Surfaces for Electron Microscopy. Biotech. Histochem. 66: 269-272.
Roberts I. M.1975: Tungsten coating- a method of improving glass microtome knives for cutting ultrathin sections. J. Microsc. 103: 113-119.
Shi S. R., Gu J., Kalra K. L., Chen T., Cote R. J., Taylor C. R.1995: Antigen Retrieval Technique: A Novel Approach to Immunohistochemistry on Routinely Processed Tissue Sections. Cell Vision 2: 7-22.
Sitte H. 1981: Ultramicrotomy-common problems and mistakes. Elektronenmikroskopie: 1-36
Skepper J.N. 2000: Immunocytochemical strategies for electron microscopy: choice or compromise. J. Microsc. 199 (Pt 1): 1-36.
Studer D. and Gnaegl H. 2000: Minimal compression of ultrathin sections with use of an oscillating diamond knife. J.Microscopy 197: 94-100.
Thiery G., Bergeron M. 1991: Influence of Calcium and Amino Acids on the Osmium Impregnation of the Endoplasmic Reticulum. J Electron Microsc. Tech. 17: 361-368.
Thomopoulos G. N., Schulte B. A., Spicer S. S. 1987: Postembedment Staining of Complex Carbohydrates: Influence of Fixation and Embedding Procedures. J. Electron Microsc. Tech. 5: 17-44.
Villiger W. and Bremer A. 1990: Ultramicrotomy of Biological Objects: From the Beginning to the Present. J. Struct. Biol. 104: 178-188.
Wang W., Cai Z., Du K. 1997: Improved methods for making and using glass knives. Biotech. Histochem. 72: 129- 134.
Weibull C., Christiansson A., Carlemalm E. 1983: Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii-cells for electron microscopy. J. Microsc.: 129: 201-207.

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Příprava preparátů pro TEM fyzikálními metodami
Robards A.W., Wilson A.J. 1993: Procedures in Electron Microscopy, Willey and Sons,Ltd.
Robinson D.G., Ehlers U., Herken R., Herrmann B., Mayer F., Schurmann F.-W. 1987: Methods of Preparation for Electron Microscopy. Spribger-Verlag, Berlin
Kok L.P., Boon M.E. 1992: Microwave Cookbook for Microscopists. Coulomb Press Leyden
Giberson R.T, Demaree Jr. R.S.2001: Microwave - Techniques and Protocols. Humana Press Totowa, New Jersey
Roos N., Morgan A.J. 1990: Cryopreparation of Thin Biological Specimens for Electron Microscopy: Methods and Applications. Oxford University Press. Royal Microscopical Society
Reid N., Beesley J.E. 1991: Sectioning and Cryosectioning for Electron Microscopy. Elsevier Amsterdam
Akisaka T.,Yoshida H., Hosoi M. 2000: Improved Preservation of Amoeba proteus Ultrastructure Revealed by Quick-Freezing Followed by Freeze-Substitution. Eur. J. Protistol. 36: 303-315.
Bert M., Menco M. P., Yankova S. A., Simon 1997: Freeze-substitution and Postembedding Immunocytochemistry on Rat Taste Buds: G-Proteins, Calcitonin Gene-related Peptide, and Choline Acetyl Transferase. Microscopy and Microanalysis 3: 53-69.
Chicoine L., Webster P. 1998: Effect of Microwave Irradiation on Antibody Labeling Efficiency when applied to Ultrathin Cryosections through Fixed Biological Material. Microsc. Res. Tech. 42: 24-32.
Demaree R. S., Giberson R. T., Smith R. L. 1995: Routine microwave polymerization of resins for transmission electron microscopy. Scanning 17 (Suppl.5): 25- 26.
Dubochet J., Sartori Blanc N. 2001: The cell in absence of aggregation artifacts. Micron 32: 91-9.
Giberson R. T., Demaree R. S. 1995: Microwave Fixation: Understaining the Variables to Achieve Rapid Reproducible Results. Microsc. Res. Tech. 32: 246-254.
Gu J., Forte M., Carson H. H. N., Xenachis C., Rufner R. 1994: Microwave fixation, antigen retrieval an accelerated immunochemistry. Cell Vision 1: 76-77.
Hippe-Sanwald S. 1993: Impact of freeze substitution on biological electron microscopy. Microsc. Res. Tech. 24: 400-422.
Horowitz R. A., Giannasca P. J., Woodcock C. L. 1990: Ultrastructural preservation of nuclei and chromatin: improvement with low-temperature methods. J. Microsc. 157:205-224.
Kellenberger E. 1991: The potential of cryofixation and freeze substitution: observations and theoretical considerations. J. Microsc. 161: 183- 203.
Keller G. A., Tokuyasu K. T., Dutton A. H., Singer S. J. 1984: An improved procedure for immunoelectron microscopy: Ultrathin plastic embedding of immunolabeled ultrathin frozen sectios. Proc. Natl. Acad. Sci. USA 81: 5744-5747.
Ryan K. P. 1992: Cryofixation of Tissues for Electron Microscopy: a Review of Plunge Cooling Methods. Scanning Microsc. 6: 715-743
Sartori Blanc N., Studer D., Ruhl K., Dubochet J. 1998: Electron beam-induced changes in vitreous sections of biological samples. J. Microsc.192: 194-201.
Steinbrecht R. A. 1993: Freeze substitution for morphological and imunologial studies in insect. Microsc. Res. Tech. 24: 488-504.
Webster P. 1993: A simple modification to the LKB 7800 series Knifemaker and a balanced-break method to prepare glass knives for cryosectioning. J. Microsc. 169: 85-88.

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Další metody přípravy preparátů pro TEM
Robards A.W., Wilson A.J. 1993: Procedures in Electron Microscopy, Willey and Sons,Ltd.
Mráz P., Polónyi J. 1988: Metody elektronovej mikroskopie živočíšnnych tkanív. Veda, Bratislava
Dawes C.J. 1988: Introduction to Biological Electron Microscopy: Theory and Techniques Ladd Research Industries, Inc. Publisher Burlington, Vermont
Rýc M., Čiampor F., Wagner M. 1989: Elektronová a imunoelektronová mikroskopie ve virologii. Avicenum, Zdravotnické nakladatelství, Praha
Robinson D.G., Ehlers U., Herken R., Herrmann B., Mayer F., Schurmann F.-W. 1987: Methods of Preparation for Electron Microscopy. Spribger-Verlag, Berlin
Boekema E. J. 1991: Negative Staining of Integral membrane proteins. Micron Microsc. Acta 22: 361-369.
Harris J. R. 1991: Negative Staining-Carbon Film Technique: New Cellular and Molecular Applications. J. Electron Microsc. Tech. 18: 269-276.
Ruigrok R. W. H., Hewat E. A. 1991: Comparison of negatively stained and frozen hydrated samples of influenza viruses A and B and of vesicular stomatitis virus. Micron Microsc. Acta 22: 423-434.
Takeuchi I. K., Takeuchi Y. K. 1990: Ethanol-Phosphotungstic Acid and Bismuth Staining of Spermatid Nucleoli in Mouse Spermiogenesis. J. Struct. Biol. 103: 104-112.

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Metody přípravy preparátů pro SEM
Robards A.W., Wilson A.J. 1993: Procedures in Electron Microscopy, Willey and Sons,Ltd.
Mráz P., Polónyi J. 1988: Metody elektronovej mikroskopie živočíšnnych tkanív. Veda, Bratislava
Dawes C.J. 1988: Introduction to Biological Electron Microscopy: Theory and Techniques Ladd Research Industries, Inc. Publisher Burlington, Vermont
Boyde A. 1991: Shrinkage in tertiary butanol prior to freeze drying. J. Electron Microsc. Tech. 19: 491-492.
Dufrene Y. F. 2001: Atomic Force Microscopy of Microbial Cells. Microsc. Analysis 70: 25-27.
Dvorak J. A., Kobayashi S., Abe K., Fujiwara T., Takeuchi T., Nagao E. 2000: The application of the atomic force microscope to studies of medically important protozoan parasites.J. Electron Microsc. 49: 429- 435.
Hermann R., Walther P., Muller M. 1996: Immunogold-labeling in SEM. Histochem. Cell. Biol. 106: 31-39.
Pěknice J. 1991: Scanning Electron Microscope. Elektronika 6: 4-5Rumph J. A., Turner W. J. 1998: Alternative to Critical Point Drying for Soft-Bodied Insect Larvae. Ann. Entomol. Soc. Am. 91: 693-699.

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Počítače a elektronová mikroskopie
Robards A.W., Wilson A.J. 1993: Procedures in Electron Microscopy, Willey and Sons,Ltd.
Hader D.P. ed., 1992: Image Analysis in Biology. CRC Press
Russ J.C. 1995: The Image Processing Handbook. CRC Press

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